Enzyme electrode for sucrose
نویسندگان
چکیده
منابع مشابه
Enzyme electrode probes.
An electrical transducer for urea is described. The excellent stability of various types of urease enzyme electrodes developed in the author's laboratory is due to understanding the parameters which affect immobilized enzyme stability. Preliminary experiments have shown that a liquid layer of urease trapped in a double cellophane layer over a cation electrode may produce a useful enzyme electro...
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An enzyme electrode was developed using the enzyme sulfite oxidase (EC1.8.3.1) immobilized onto pig intestine. Hydrogen peroxide produced was monitored amperometrically. All experimental parameters such as pH, temperature, buffer constituent concentration, were thoroughly investigated and optimized when appropriate. The response to sulfite concentration was linear in the range 5.2´...
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An efficient catalyst based on artificial bionic peroxidase was synthesized for electrocatalysis. A poly(ethyleneimine)/Au nanoparticle composite (PEI-AuNP) was prepared and it was then linked to hemin via a coupling reaction between carboxyl groups in hemin and amino groups in PEI without the activation of a carboxyl group by carbodiimide. Fourier transform infrared (FTIR) spectroscopy verifie...
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A nanoporous membrane system with directed flow carrying reagents to sequentially attached enzymes to mimic nature’s enzyme complex system was demonstrated. Genetically modified glycosylation enzyme, OleD Loki variant, was immobilized onto nanometer-scale electrodes at the pore entrances/exits of anodic aluminum oxide membranes through His6-tag affinity binding. The enzyme activity was assessed...
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Coimmobilization of beta-galactosidase and glucose oxidase in a redox polymer, polyvinylferrocenium perchlorate (PVF+ ClO4-), led to the development of an enzyme electrode for the determination of lactose. The amperometric response of the electrode was measured at +0.70 V vs. SCE, which was due to the electrooxidation of enzymatically produced H2O2. The effects of the substrate and buffer conce...
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ژورنال
عنوان ژورنال: Biotechnology and Bioengineering
سال: 1976
ISSN: 0006-3592,1097-0290
DOI: 10.1002/bit.260180211